Today's post from sciencedaily.com (see link below) revisits an area of research that seems to have been relatively quiet recently and that is the potential for animal venoms to create pain killers. This time, the article talks about Tarantula spider toxins and in particular the exotic sounding, Peruvian green velvet tarantula. Apparently this creature's venom can selectively inhibit pain receptors and as such, when refined, has great potential as a future pain killer, especially for nerve pain. When you think that the spider's purpose is to paralyse its prey so that there's no struggle and easy eating, then the stretch of our imagination may not be so great. The article becomes more complex as it goes on but you will get the gist and we can once again be excited by the potential of snake, spider and fish venoms to help relieve our neuropathy symptoms. The sooner it can be put into a bottle, the sooner we won't be confronted by images of its donor!
Date:February 29, 2016Source:Biophysical Society
It turns out that peptide toxins isolated from the venom of some animals -- such as the Peruvian green velvet tarantula -- can be beneficial when used to target neural receptors to reduce the sensation of pain.
When venom from animals such as spiders, snakes or cone snails is injected via a bite or harpoon, the cocktail of toxins delivered to its victim tends to cause serious reactions that, if untreated, can be lethal. But even venom has a therapeutic upside: Individual peptide toxins are being tapped to target receptors in the brain to potentially serve as painkillers.
Millions of people live with chronic and neuropathic pain, in large part because current treatments often provide limited pain relief, have a heavy profile of soporific side effects and can be extremely addictive. So researchers around the globe are chasing down potential new therapeutic agents and working to gain a better understanding of how molecules with painkiller activity function. This will lead to alternative painkillers--and possibly improve the quality of life for people who suffer from chronic pain.
At the Biophysical Society's 60th Annual Meeting, being held in Los Angeles, Calif., Feb. 27-March 2, 2016, a group of researchers from the University of Queensland in Brisbane, Australia, will describe their efforts with ProTx-II, a peptide toxin found within the venom of the Peruvian green velvet tarantula, Thrixopelma pruriens. Its high potency and selectivity to inhibit the pain sensation receptor make it an ideal candidate as a future painkiller.
"Our group is specifically interested in understanding the mode of action of this toxin to gain information that can guide us in the design and optimization of novel pain therapeutics," said Sónia Troeira Henriques, senior research officer at the University of Queensland's Institute for Molecular Bioscience.
How does ProTx-II work? "It binds to the pain receptor located within the membrane of neuronal cells, but the precise peptide-receptor binding site and the importance of the cell membrane in the inhibitory activity of ProTx-II is unknown," explained Henriques.
So the group zeroed in on its structure-activity relationship by "exploring the structure, the membrane-binding properties, and the inhibitory activity of ProTx-II and a series of analogues," she added.
Nuclear magnetic resonance (NMR) spectroscopy enables 3-D characterization of the structure of this peptide, which allows the group to explore whether it's important for its ability to inhibit the pain receptor.
They also use surface plasmon resonance and fluorescence methodologies, as well as molecular simulations, to further characterize the interactions between the peptide and the neuronal cell membrane and to identify the molecular properties of the peptide involved in the interaction and inhibition with the pain receptor.
"Our results show that the cell membrane plays an important role in the ability of ProTx-II to inhibit the pain receptor. In particular, the neuronal cell membranes attract the peptide to the neurons, increase its concentration close to the pain receptors, and lock the peptide in the right orientation to maximize its interaction with the target," said Henriques.
The group's work is the first to describe the importance of the membrane-binding properties of ProTx-II for its potency as an inhibitor of Nav 1.7, an important pain receptor. "Until now, studies characterizing the inhibitory activity of venom toxins have ignored the potential role of the cell membrane in their potency and activity," she noted.
Beyond Nav 1.7, "other voltage-gated ion channels are located at the cell membrane and involved in a range of physiological processes such as muscle and nerve relaxation, regulation of blood pressure, and sensory transduction," Henriques pointed out. "Their 'faulty' activity is, however, associated with several disorders, so other ion channels are actively being pursued as drug targets for the treatment of neuromuscular disease, neurological disorders, and inflammatory and neuropathic pain."
Based on the group's findings, they're now designing new toxins with greater affinity for the cell membrane and fewer side effects.
"Our work creates an opportunity to explore the importance of the cell membrane in the activity of peptide toxins that target other voltage-gated ion channels involved in important disorders," said Henriques.
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The above post is reprinted from materials provided by Biophysical Society. Note: Materials may be edited for content and length.
https://www.sciencedaily.com/releases/2016/02/160229082005.htm
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